Mouse Osteonectin / SPARC
(tested or 100% immunogen sequence identity)
Protein G purified
- IHC - Frozen (25 µg/ml)
- Flow Cytometry (25 µg/ml)
- ELISA (0.5 - 1 µg/ml)
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Recombinant mouse Osteonectin.
Recognizes mouse Osteonectin. Displays no cross-reactivity with recombinant human Osteonectin.
Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. ELISA: 0.5-1 ug/ml). Immunohistochemistry (frozen): 25 ug/ml detects SPARC on mouse ovary tissue. Flow Cytometry: 25 ug/ml. For intracellular staining, cells must be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin. Dilute this antibody to 25 ug/mL and add 10uL of this solution to 1-5x10^5 cells in a total reaction volume 200uL. Following a 30 minute incubation, cells should be washed with 0.1% saponin prior to adding 10 ml of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis.
Lyophilized from PBS, pH 7.4, 5% trehalose
Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C. Further dilutions can be made in buffer.
For research use only.
This antibody carries the LSBio 100% Guarantee.