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Anti-Osteonectin / SPARC Antibody LS-C124320

Ordering

Wt. Vol. Conc. Price
500 µg - 0.5 mg/ml $650
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Osteonectin / SPARC Antibodies

Anti-Osteonectin / SPARC Antibody (clone 5031) LS-C25800
Mouse Monoclonal [clone 5031] (IgG1) to Human Osteonectin / SPARC
Human, Rat, Bovine
IHC, Western blot, ELISA, Radioimmunoassay
Unconjugated
Anti-Osteonectin / SPARC Antibody (aa1-304, Biotin) LS-C280453
Mouse Monoclonal (IgG1) to Human Osteonectin / SPARC
Human
Western blot
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Rat Monoclonal (IgG2b) to Mouse Osteonectin / SPARC
Mouse
IHC - Frozen, Flow Cytometry, ELISA
Unconjugated

Details

Mouse Osteonectin / SPARC
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG2b Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Frozen (25 µg/ml)
  • Flow Cytometry (25 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

Osteonectin / SPARC antibody was raised against recombinant mouse Osteonectin.
Recognizes mouse Osteonectin. Displays no cross-reactivity with recombinant human Osteonectin.
Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. ELISA: 0.5-1 ug/ml). Immunohistochemistry (frozen): 25 ug/ml detects SPARC on mouse ovary tissue. Flow Cytometry: 25 ug/ml. For intracellular staining, cells must be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin. Dilute this antibody to 25 ug/mL and add 10uL of this solution to 1-5x10^5 cells in a total reaction volume 200uL. Following a 30 minute incubation, cells should be washed with 0.1% saponin prior to adding 10 ml of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis.

Packaging

Lyophilized from PBS, pH 7.4, 5% trehalose
Sterile PBS
Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Further dilutions can be made in buffer.
For research use only.

About Osteonectin / SPARC

P09486 NM_003118 NP_003109.1

SPARC Antibody, Basement-membrane protein 40 Antibody, BM-40 Antibody, Cysteine-rich protein Antibody, ON Antibody, Osteonectin Antibody

SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules.

Requested From: United States
Date Requested: 12/3/2016

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