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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Osteonectin / SPARC Antibody LS‑C124320
Antibody
SPARC antibody LS-C124320 is an unconjugated rat monoclonal antibody to mouse SPARC (Osteonectin). Validated for ELISA, Flow and IHC.
Catalog
Size
Price
LS-C124320-500
500 µg (0.5 mg/ml)
Unavailable

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Product Description

SPARC antibody LS-C124320 is an unconjugated rat monoclonal antibody to mouse SPARC (Osteonectin). Validated for ELISA, Flow and IHC.
About Osteonectin / SPARC
SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules. P09486 NM_003118 NP_003109.1

SPARC Antibody, Basement-membrane protein 40 Antibody, BM-40 Antibody, Cysteine-rich protein Antibody, ON Antibody, Osteonectin Antibody


Specifications

Target
Mouse Osteonectin / SPARC
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen (25 µg/ml)
  • Flow Cytometry (25 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant mouse Osteonectin.
Specificity
Recognizes mouse Osteonectin. Displays no cross-reactivity with recombinant human Osteonectin.
Usage
Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. ELISA: 0.5-1 ug/ml). Immunohistochemistry (frozen): 25 ug/ml detects SPARC on mouse ovary tissue. Flow Cytometry: 25 ug/ml. For intracellular staining, cells must be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin. Dilute this antibody to 25 ug/mL and add 10uL of this solution to 1-5x10^5 cells in a total reaction volume 200uL. Following a 30 minute incubation, cells should be washed with 0.1% saponin prior to adding 10 ml of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis.
Presentation
Lyophilized from PBS, pH 7.4, 5% trehalose
Reconstitution
Sterile PBS
Storage
Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C. Further dilutions can be made in buffer.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/24/2018

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