Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG1,k) to Human Osteonectin / SPARC
Mouse Monoclonal [clone ON1-1] (IgG1) to Bovine Osteonectin / SPARC
IHC - Paraffin, IHC - Frozen, Western blot
Bovine Osteonectin / SPARC
Bovine, Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [ON1-1]
IHC - Paraffin (1 - 10 µg/ml)
IHC - Frozen (1 - 10 µg/ml)
Western blot (1 - 10 µg/ml)
Specificity and Use
Bovine bone osteonectin.
Reacts with human and bovine osteonectin as determined by Western Blot, and cross-reacts with rabbit and porcine osteonectin as determined by ELISA. Reacts with both bone and platelet derived osteonectin; it does not react with non-activated platelets, but it does react with thrombin-stimulated platelets, as determined by Flow Cytometry.
Western Blot: Reducing and non-reducing conditions.
Lyophilized from 10 mM PBS, pH 7.4 with 1% BSA
50 µl Distilled Water.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
SPARC Antibody, Basement-membrane protein 40 Antibody, BM-40 Antibody, Cysteine-rich protein Antibody, ON Antibody, Osteonectin Antibody
SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules.