Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone LM2] (IgG1) to Human Osteonectin / SPARC
IHC - Paraffin, Western blot, Flow Cytometry
Human Osteonectin / SPARC
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [LM2]
Protein A/G purified
IHC - Paraffin
Specificity and Use
Osteonectin / SPARC antibody was raised against recombinant fragment of human SPARC.
Antibody can be used for immunohistochemistry at 10-20 ug/ml on paraffin-embedded tissue sections. Can also be used for flow cytometry at 3 g/10^6 cells. Optimal concentration should be evaluated by serial dilutions.
PBS, 0.08% Sodium Azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
SPARC Antibody, Basement-membrane protein 40 Antibody, BM-40 Antibody, Cysteine-rich protein Antibody, ON Antibody, Osteonectin Antibody
SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules.