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Anti-Osteonectin / SPARC Antibody (aa224-251) LS-C161424

Catalog Size Price
LS-C161424-400 400 µl Unavailable

Most Popular Osteonectin / SPARC Antibodies

Anti-Osteonectin / SPARC Antibody (clone 5031) LS-C25800
Mouse Monoclonal [clone 5031] (IgG1) to Human Osteonectin / SPARC
Human, Rat, Bovine
IHC, Western blot, ELISA, Radioimmunoassay
Unconjugated
Anti-Osteonectin / SPARC Antibody (Gly53) LS-C191122
Rabbit Polyclonal (IgG) to Human Osteonectin / SPARC
Human, Monkey, Mouse
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Anti-Osteonectin / SPARC Antibody (C-Terminus, Biotin) LS-C280447
Rabbit Polyclonal (IgG) to Human Osteonectin / SPARC
Human
IHC, Western blot, Flow Cytometry
Biotin Conjugated
Anti-Osteonectin / SPARC Antibody (aa1-304, Biotin) LS-C280453
Mouse Monoclonal (IgG1,k) to Human Osteonectin / SPARC
Human
Western blot
Biotin Conjugated
Anti-Osteonectin / SPARC Antibody (C-Terminus) IHC-plus™ LS-B11553
Rabbit Polyclonal to Human Osteonectin / SPARC
Human, Monkey, Mouse, Rat, Horse, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Rabbit Polyclonal to Human Osteonectin / SPARC
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human Osteonectin / SPARC
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Pig, Rabbit, Chicken, Xenopus, Cow (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E8432 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C161424. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa224-251
This SPARC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About Osteonectin / SPARC

P09486 NM_003118 NP_003109.1

SPARC Antibody, Basement-membrane protein 40 Antibody, BM-40 Antibody, Cysteine-rich protein Antibody, ON Antibody, Osteonectin Antibody

SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human skin tissue reacted with SPARC Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot
Western blot of SPARC (arrow) using rabbit polyclonal SPARC Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPARC gene.

Western blot

Western blot
Western blot of SPARC Antibody in Y79 cell line lysates (35 ug/lane). SPARC (arrow) was detected using the purified antibody.

Flow Cytometry

Flow Cytometry
Flow cytometric of widr cells using SPARC Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 8/21/2017

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