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Anti-Osteonectin / SPARC Antibody (aa18-302) LS-C150257


Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular Osteonectin / SPARC Antibodies

Anti-Osteonectin / SPARC Antibody (clone 5031) LS-C25800
Mouse Monoclonal [clone 5031] (IgG1) to Human Osteonectin / SPARC
Human, Rat, Bovine
IHC, Western blot, ELISA, Radioimmunoassay
Anti-Osteonectin / SPARC Antibody (aa1-304, Biotin) LS-C280453
Mouse Monoclonal (IgG1) to Human Osteonectin / SPARC
Western blot
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Mouse Osteonectin / SPARC
IHC, Western blot, Flow Cytometry


Mouse Osteonectin / SPARC
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • Flow Cytometry

Specificity and Use

Osteonectin / SPARC antibody was raised against mouse myeloma cell line NS0-derived recombinant mouse SPARC/Osteonectin Ala18-Ile302 Accession # P07214.
Detects mouse SPARC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1 % cross-reactivity with.
Western Blot, 0.1 µg/ml, Recombinant Mouse SPARC/Osteonectin. Immunohistochemistry, 5-15 µg/ml, Intracellular Staining by Flow Cytometry, 2.5 µg/106 cells, Balb/3T3 mouse embryonic fibroblast cell line fixed with paraformaldehyde and permeabilized. with saponin.


Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About Osteonectin / SPARC

P09486 NM_003118 NP_003109.1

SPARC Antibody, Basement-membrane protein 40 Antibody, BM-40 Antibody, Cysteine-rich protein Antibody, ON Antibody, Osteonectin Antibody

SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules.


SPARC/Osteonectin in Mouse Embryo. SPARC/Osteonectin was detected in immersion fixed frozen sections of mouse embryo (E15) using Mouse SPARC/Osteonectin Antigen Affinity-purified Polyclonal Antibody at 1.7 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing cartilage.

Requested From: United States
Date Requested: 10/25/2016

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