LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-Osteocalcin Antibody (aa1-20) LS-C17044

Ordering

Wt. Vol. Conc. Price
100 µg 50 µl 2 mg/ml $920
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Osteocalcin Antibodies

Anti-Osteocalcin Antibody (C-Terminus) LS-C42094
Goat Polyclonal (IgG) to Mouse Osteocalcin
Mouse
IHC - Frozen
Unconjugated
Anti-Osteocalcin Antibody (aa1-20) LS-C17044
Rabbit Polyclonal (IgG) to Mouse Osteocalcin
Mouse
IHC - Paraffin
Unconjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal (IgG) to Mouse Osteocalcin
Mouse
IHC - Paraffin
Unconjugated

Details

Mouse Osteocalcin
Rabbit
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

IHC - Paraffin (1 - 2 µg/ml)
Western blot

Specificity and Use

Osteocalcin antibody was raised against synthetic peptide corresponding to aa1-20 (YLGASVPSPDPLEPTREQCE) of intracellular N-terminal mouse osteocalcin. Percent identity by BLAST analysis: Mouse (100%).
aa1-20
Recognizes intracellular N-terminal mouse osteocalcin. Does not cross-react with rat osteocalcin.
Suitable for use in Immunohistochemistry. Not suitable for use in Western Blot. Immunohistochemistry (Paraffin sections): 1-2 ug/ml. Trypsin treatment recommended for antigen retrieval. "Osteocalcin was detected on formalin-fixed, paraffin-embedded sections. Briefly, slides were heated at 55°C for 15 min, deparaffinized in two washes of xylene, and rehydrated in a decreasing ethanol gradient. Antigen retrieval was performed using 0.5% trypsin for 30 min at 37°C in a humidified chamber. Endogenous peroxidase was deactivated with 3% H2O2 for 30 min, and sections were blocked in PBS supplemented with 0.1% Triton-X, 2% goat serum, and 1% BSA for 20 min at RT. Sections were incubated with primary antibody (1:200 dilution) in blocking solution overnight at 4°C. Sections were then washed in PBS and incubated with a IgG (HRP) Pab donkey x rabbit secondary antibody in PBS for 45 min at RT. After washing with PBS, HRP activity was detected using a DAB substrate kit according to the manufacturer's instructions."

Packaging

Lyophilized, 10mM PBS, pH 7.4, 1% BSA
50 µl 50ul sterile ddH2O, 0.1% sodium azide
Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.
For research use only.

Publications (1)

1
Expression of metastasin S100A4 is essential for bone resorption and regulates osteoclast function. Erlandsson MC, Svensson MD, Jonsson IM, Bian L, Ambartsumian N, Andersson S, Peng Z, V[Character e4]r[Character e4]niemi J, Ohlsson C, Andersson KM, Bokarewa MI. Biochimica et biophysica acta. 2013 Dec;1833:2653-63. [Full Text Article] [PubMed:23830916]

About Osteocalcin

P02818 BG752923 NP_954642.1

BGLAP Antibody, Bone Gla protein Antibody, BGP Antibody, OC Antibody, Osteocalcin Antibody

Osteocalcin (BGLAP) is secreted by osteoblasts and plays a role in metabolic regulation. It is pro-osteoblastic (bone-building). It is also implicated in bone mineralization and calcium ion homeostasis. Osteocalcin acts as a hormone in the body, causing beta cells in the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin.

Requested From: United States
Date Requested: 12/8/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number