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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3065-125 | 125 µl | $575 |
IHC‑plus™ Polyclonal Rabbit anti‑Human OPTN / Optineurin Antibody (aa559‑575, IHC, WB) LS‑B3065
IHC‑plus™ Polyclonal Rabbit anti‑Human OPTN / Optineurin Antibody (aa559‑575, IHC, WB) LS‑B3065
Note: This antibody replaces LS-C17028
Antibody:
OPTN / Optineurin Rabbit anti-Human Polyclonal (aa559-575) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
OPTN / Optineurin Rabbit anti-Human Polyclonal (aa559-575) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
Optineurin antibody LS-B3065 is an unconjugated rabbit polyclonal antibody to Optineurin (OPTN) (aa559-575) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human OPTN / Optineurin
Synonyms
OPTN | ALS12 | E3-14.7K-interacting protein | FIP-2 | HIP-7 | HYPL | Huntingtin yeast partner L | HIP7 | TFIIIA-INTP | NEMO-related protein | NRP | Optineurin | FIP2 | GLC1E
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to aa 559-575 (GEVLPDIDTLQIHVMDC) of human, mouse and rat optineurin
Epitope
aa559-575
Specificity
Recognizes human, mouse and rat optineurin.
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Western blot (1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3065 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3065 was determined to be 2.5 ug/ml. Western Blot: 1:200. Incubation time of 1 hour at RT. Overnight incubations at 4uC at lower concentrations can produce optimal results.
Presentation
TBS, pH 7.4, 0.5 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About OPTN / Optineurin
Validation
Anti-Optineurin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Optineurin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ OPTN / Optineurin Antibody (aa559-575) for IHC, WB/Western LS-B3065 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Monkey
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Mouse, Rat, Bat, Dog, Horse, Rabbit
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
Western blot, ELISA
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Source:
Yeast
Tag:
6His, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
