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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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OPTC / Opticin Antibody (aa254‑303) LS‑C81989
Opticin antibody LS-C81989 is an unconjugated rabbit polyclonal antibody to Opticin (OPTC) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C81989-50
50 µg
$365

Popular OPTC / Opticin Products

Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human, Mouse, Rat
Applications: Western blot
Western blot analysis of Opticin expression in HeLa (A); mouse heart (B); rat heart (C) whole cell lysates.
Species: Human, Mouse, Rat, Pig
Applications: Western blot
Antibody
Species: Human, Mouse, Rat
Applications: Western blot
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

Opticin antibody LS-C81989 is an unconjugated rabbit polyclonal antibody to Opticin (OPTC) from human, mouse, rat and other species. Validated for WB.
About OPTC / Opticin
Opticin belongs to class III of the small leucine-rich repeat protein (SLRP) family. Members of this family are typically associated with the extracellular matrix. Opticin is present in significant quantities in the vitreous of the eye and also localizes to the cornea, iris, ciliary body, optic nerve, choroid, retina, and fetal liver. Opticin may noncovalently bind collagen fibrils and regulate fibril morphology, spacing, and organization. Q9UBM4 NM_014359 NP_055174.1

OPTC Antibody, Oculoglycan Antibody, OPT Antibody, Opticin Antibody


Specifications

Target
Human OPTC / Opticin
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog, Guinea pig, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
OPTC / Opticin antibody was raised against synthetic peptide located between aa254-303 of human OPTC (Q9UBM4, NP_055174). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Rat, Panda, Dog, Pig (100%); Rabbit, Horse (92%); Galago, Mouse, Guinea pig (84%).
Blocking Peptide
LS-E19863 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C81989. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa254-303
Specificity
Human OPTC
Usage
ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 8/16/2018

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