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Anti-OPG / Osteoprotegerin Antibody LS-C104334

Note: This antibody replaces LS-C54249, LS-C187916, LS-C17052

Ordering

Wt. Vol. Conc. Price
50 µg - - $285
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular OPG / Osteoprotegerin Antibodies

Anti-OPG / Osteoprotegerin Antibody (aa20-37, clone 98A1071) IHC-plus™ LS-B2078
Mouse Monoclonal [clone 98A1071] (IgG) to Human OPG / Osteoprotegerin
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-OPG / Osteoprotegerin Antibody (aa1-50) IHC-plus™ LS-B11213
Rabbit Polyclonal to Human OPG / Osteoprotegerin
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal to Human OPG / Osteoprotegerin
Human
IHC, Western blot, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Human OPG / Osteoprotegerin
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

OPG / Osteoprotegerin antibody was raised against e.coli derived recombinant Human OPG.
Human OPG
Immunohistochemistry: To detect Human OPG by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with LifeSpan's Biotinylated Anti-Human OPG (LS-C104334) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human OPG. ELISA: To detect hOPG by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human OPG (LS-C104333) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hOPG. Western Blot: To detect hOPG by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOPG is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About OPG / Osteoprotegerin

O00300 NM_002546 NP_002537.3

TNFRSF11B Antibody, OCIF Antibody, OPG Antibody, Osteoprotegerin Antibody, TR1 Antibody

Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.

Immunohistochemistry

Immunohistochemistry
IHC of Osteoprotegerin / OPG antibody LS-C104334. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
IHC of Osteoprotegerin / OPG antibody LS-C104334. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of Osteoprotegerin / OPG antibody LS-C104334. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/10/2016

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