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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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OPA3 Antibody (aa95‑144) LS‑C135626
OPA3 antibody LS-C135626 is an unconjugated rabbit polyclonal antibody to human OPA3. Validated for WB.
Catalog
Size
Price
LS-C135626-50
50 µg
Unavailable

Popular OPA3 Products

Immunohistochemical analysis of OPA3 staining in human gastric cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Applications: IHC, IHC - Paraffin, Western blot
Western Blot; Sample: Recombinant protein.
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Immunohistochemistry of paraffin-embedded human kidney tissue at dilution 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue at dilution 1:100
Species: Human
Applications: IHC, Immunofluorescence, ELISA

Product Description

OPA3 antibody LS-C135626 is an unconjugated rabbit polyclonal antibody to human OPA3. Validated for WB.
About OPA3
The mouse ortholog of this protein co-purifies with the mitochondrial inner membrane. Mutations in this gene have been shown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract. Multiple transcript variants encoding different isoforms have been found for this gene. Q9H6K4 NM_025136 NP_079412.1

OPA3 Antibody, MGA3 Antibody, Optic atrophy 3 protein Antibody


Specifications

Target
Human OPA3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
OPA3 antibody was raised against synthetic peptide located between aa95-144 of human OPA3 (Q9H6K4, NP_001017989). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%); Marmoset (90%); Monkey (85%).
Blocking Peptide
LS-E21895 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135626. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa95-144
Specificity
Human OPA3
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

OPA3 antibody LS-C135626 Western blot of Fetal Kidney lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
OPA3 antibody LS-C135626 Western blot of Fetal Kidney lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

OPA3 antibody LS-C135626 Western blot of Fetal Kidney lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
OPA3 antibody LS-C135626 Western blot of Fetal Kidney lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

OPA3 antibody LS-C135626 Western blot of Fetal Kidney lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
OPA3 antibody LS-C135626 Western blot of Fetal Kidney lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

OPA3 antibody LS-C135626 Western blot of Fetal Kidney lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
OPA3 antibody LS-C135626 Western blot of Fetal Kidney lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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