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Anti-OCLN / Occludin Antibody (C-Terminus) IHC-plus™ LS-B2187

Note: This antibody replaces LS-C37847


Wt. Vol. Conc. Price
50 µg - 0.25 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular OCLN / Occludin Antibodies

Anti-OCLN / Occludin Antibody (C-Terminus) IHC-plus™ LS-B2187
Rabbit Polyclonal (IgG) to Human OCLN / Occludin
Human, Mouse, Rat, Dog
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Immunohistochemistry Image
Anti-OCLN / Occludin Antibody (C-Terminus, clone OC-3F10) IHC-plus™ LS-B2320
Mouse Monoclonal [clone OC-3F10] (IgG1,k) to Human OCLN / Occludin
Human, Mouse, Rat, Dog
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Image
Anti-OCLN / Occludin Antibody (aa35-84) IHC-plus™ LS-B5737
Rabbit Polyclonal to Human OCLN / Occludin
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal (IgG) to Human OCLN / Occludin
Human, Mouse, Rat, Dog
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA


Human OCLN / Occludin
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)

Specificity and Use

OCLN / Occludin antibody was raised against a GST fusion protein consisting of the C-terminal 150 amino acids of human occludin fused to GST.
Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.
Immunohistochemistry: LS-B2187 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2187 was determined to be 2.5 ug/ml.


PBS, pH 7.4, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (2)

The tight-junction protein claudin-6 induces epithelial differentiation from mouse F9 and embryonic stem cells. Sugimoto K, Ichikawa-Tomikawa N, Satohisa S, Akashi Y, Kanai R, Saito T, Sawada N, Chiba H. PloS one. 2013 8:e75106. [Full Text Article] [PubMed:24116027] [PMC:PMC3792957]
ATF3 Protects Pulmonary Resident Cells from Acute and Ventilator-Induced Lung Injury by Preventing Nrf2 Degradation. Shan Y, Akram A, Amatullah H, Zhou DY, Gali PL, Maron-Gutierrez T, Gonz[Character e1]lez-L[Character f3]pez A, Zhou L, Rocco PR, Hwang D, Albaiceta GM, Haitsma JJ, Dos Santos CC. Antioxidants & redox signaling. 2014 Mar;22:651-68. [Full Text Article] [PubMed:25401197] [PMC:PMC4346377]

About OCLN / Occludin

Q16625 NM_002538 NP_002529.1

OCLN Antibody, Occludin Antibody, BLCPMG Antibody

OCLN / Occludin is an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants.


Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2187 concentration 5 ug/ml.

Requested From: United States
Date Requested: 10/23/2016

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