NUP153 antibody LS-C9358 is an unconjugated mouse monoclonal antibody to NUP153 from human, mouse, rat and other species. Validated for IF, IHC, IP and WB.
Rat, Human, Mouse, Xenopus, Rodent
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- IHC - Frozen
- Western blot (1:30 - 1:50)
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NUP153 antibody was raised against nuclear matrix protein fraction prepared from rat liver nuclei.
Recognizes the nuclear pore complex (NPC) protein nup153 (190kD), present on the inner aspect of NPC with attached filament bundles. It is also reactive with the soluble form of nup153 found e. g. during meiotic metaphase. Species cross-reactivity: Human, rodents (mouse, rat), marsupials (kangaroo rat), amphibia (xenopus, pleurodeles).
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence microscopy and Immunohistochemistry. Immunohistochemistry: 1:5-1:10; Incubation time 1 hr at RT (frozen sections; also after fixation with paraformaldehyde). Western Blot: 1:30-1:50 (ECL method).
0.09% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.