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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NUP153 Antibody LS‑C9358 Best Seller
NUP153 antibody LS-C9358 is an unconjugated mouse monoclonal antibody to NUP153 from human, mouse, rat and other species. Validated for IF, IHC, IP and WB.
Antibody
Catalog
Size
Price
LS-C9358-5000
5000 µl
$605

Popular NUP153 Products

Immunofluorescence showing Nup 153 in HeLa cells
Species: Human, Rat, Xenopus
Applications: Immunofluorescence, Western blot, Immunoprecipitation
NUP153 antibody LS-C145463 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey
Applications: Western blot
Detection of Human NUP153 by Immunocytochemistry. Sample: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-NUP153 used at a dilution of 1:250. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.
Species: Human
Applications: ICC, Immunofluorescence
Detection of Human NUP153 by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-NUP153 used at a dilution of 1:200 (1 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

NUP153 antibody LS-C9358 is an unconjugated mouse monoclonal antibody to NUP153 from human, mouse, rat and other species. Validated for IF, IHC, IP and WB.
About NUP153
Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. P49790 NM_005124 NP_005115.2

NUP153 Antibody, 153 kDa nucleoporin Antibody, N153 Antibody, Nucleoporin 153kD Antibody, Nucleoporin 153kDa Antibody, HNUP153 Antibody, Nucleoporin Nup153 Antibody


Specifications

Target
Rat NUP153
Host
Mouse
Reactivity
Rat, Human, Mouse, Xenopus, Rodent (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Immunofluorescence
  • Western blot (1:30 - 1:50)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NUP153 antibody was raised against nuclear matrix protein fraction prepared from rat liver nuclei.
Specificity
Recognizes the nuclear pore complex (NPC) protein nup153 (190kD), present on the inner aspect of NPC with attached filament bundles. It is also reactive with the soluble form of nup153 found e. g. during meiotic metaphase. Species cross-reactivity: Human, rodents (mouse, rat), marsupials (kangaroo rat), amphibia (xenopus, pleurodeles).
Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence microscopy and Immunohistochemistry. Immunohistochemistry: 1:5-1:10; Incubation time 1 hr at RT (frozen sections; also after fixation with paraformaldehyde). Western Blot: 1:30-1:50 (ECL method).
Presentation
0.09% sodium azide.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 7/20/2018

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