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Anti-NUP153 Antibody LS-C9358

Ordering

Wt. Vol. Conc. Price
- 5000 µl - $475
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Mouse Monoclonal (IgG1) to Rat NUP153
Rat, Human, Mouse, Xenopus, Rodent
IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

Rat NUP153
Mouse
Rat, Human, Mouse, Xenopus, Rodent (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Tissue culture supernatant

Applications

IHC - Frozen, Immunofluorescence, Western blot (1:30 - 1:50), Immunoprecipitation

Specificity and Use

NUP153 antibody was raised against nuclear matrix protein fraction prepared from rat liver nuclei.
Recognizes the nuclear pore complex (NPC) protein nup153 (190kD), present on the inner aspect of NPC with attached filament bundles. It is also reactive with the soluble form of nup153 found e. g. during meiotic metaphase. Species cross-reactivity: Human, rodents (mouse, rat), marsupials (kangaroo rat), amphibia (xenopus, pleurodeles).
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence microscopy and Immunohistochemistry. Immunohistochemistry: 1:5-1:10; Incubation time 1 hr at RT (frozen sections; also after fixation with paraformaldehyde). Western Blot: 1:30-1:50 (ECL method).

Packaging

0.09% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About NUP153

P49790 NM_005124 NP_005115.2

NUP153 Antibody, 153 kDa nucleoporin Antibody, N153 Antibody, Nucleoporin 153kD Antibody, Nucleoporin 153kDa Antibody, HNUP153 Antibody, Nucleoporin Nup153 Antibody

Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner.

Requested From: United States
Date Requested: 9/30/2016

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