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Anti-NUMB Antibody (clone 291319) LS-C150019


Wt. Vol. Conc. Price
100 µg - - $315
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular NUMB Antibodies

Anti-NUMB Antibody (C-Terminus) IHC-plus™ LS-B7106
Rabbit Polyclonal to Human NUMB
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-NUMB Antibody (aa1-50) IHC-plus™ LS-B8003
Rabbit Polyclonal to Human NUMB
Human, Monkey, Mouse, Rat, Bovine
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 291319] (IgG2b) to Human NUMB
ICC, Western blot


Human NUMB
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [291319]
Protein G purified


  • ICC (10 µg/ml)
  • Western blot (0.1 µg/ml)

Specificity and Use

NUMB antibody was raised against e. coli-derived rhNumb.
Human Numb Immunogen: E. coli-derived rhNumb.
Western blot-An antibody concentration of 0.1 µg/ml is recommended. Immunocytochemistry-This antibody was used at a concentration of 10 µg/ml to detect NUMB in Hela human cervical carcinoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by fluorochrome-coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA.


Lyophilized from 0.2 um filtered solution in PBS, 5% trehalose
Sterile PBS. Resuspend to 200 ul
-20°C for one year lyophilized.
For research use only.

About NUMB

P49757 NM_003744 NP_003735.3

NUMB Antibody, C14_5527 Antibody, Numb (Drosophila) homolog Antibody, S171 Antibody, Protein numb homolog Antibody, C14orf41 Antibody, H-Numb Antibody, Numb homolog (Drosophila) Antibody, Protein S171 Antibody

Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity.

Western blot

Western blot
Detection of Numb. Lysates from human A549, HeLa and U2-OS cells were resolved by SDS-PAGE, transferred to a PVDF membrane and immunoblotted with 0.1 ug/ml mouse anti-Numb.

Requested From: United States
Date Requested: 10/25/2016

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