Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation
IHC - Paraffin
IHC - Frozen
ICC (1:20 - 1:30)
Immunofluorescence (1:10 - 1:30)
Specificity and Use
Histone specific; stains nuclei and chromosomes of cells of all species including animals and plants. Reacts preferentially with Histone H1 by ELISA and immunoblot. Produces a speckled nuclear staining pattern in all tissues studies. Stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Nuclear staining in interphase cells is intense and diffuse.
Suitable for use in ELISA, Immunocytochemistry, Indirect Immunofluorescence and Immunohistochemistry. Immunocytochemistry (Indirect immunofluorescence): 1:20-1:30, 30-100 ul per slide/well using paraformaldehyde/acetone fixed cell preparation. Immunohistochemistry: Formalin fixed, paraffin embedded tissue sections or paraformaldehyde/acetone fixed frozen tissue sections. Positive control: Human tonsil.