Human Nuclear Spliceosomes
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- Immunofluorescence (1:20 - 1:30)
- Western blot
Stains human nuclear spliceosomes. Using indirect immunofluorescence, produces a speckled nuclear staining patter. Immunoelectron microscopy has shown that the antibody recognizes a discrete area of the nucleus identified as interchromatin granules. Western Blots 105kD molecular weight protein from human cells. Immunoprecipitates spliceosome and level sn RNPs 1,2,5 (using 0.5 M salt).
Suitable for use in Indirect Immunofluorescence, Western Blot. Indirect immunofluorescence: 1:20-1:30. Use 30-100 ul per well/slide.
PBS, 0.1% sodium azide.
Short term: 4°C. Long term: Aliquot and add glycerol (40-50%). Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.