Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgM) to Human Nuclear Spliceosomes
Immunofluorescence, Western blot
Human Nuclear Spliceosomes
Human (tested or 100% immunogen sequence identity)
Tissue culture supernatant
Immunofluorescence (1:20 - 1:30)
Specificity and Use
Stains human nuclear spliceosomes. Using indirect immunofluorescence, produces a speckled nuclear staining patter. Immunoelectron microscopy has shown that the antibody recognizes a discrete area of the nucleus identified as interchromatin granules. Western Blots 105kD molecular weight protein from human cells. Immunoprecipitates spliceosome and level sn RNPs 1,2,5 (using 0.5 M salt).
Suitable for use in Indirect Immunofluorescence, Western Blot. Indirect immunofluorescence: 1:20-1:30. Use 30-100 ul per well/slide.
PBS, 0.1% sodium azide.
Short term: 4°C. Long term: Aliquot and add glycerol (40-50%). Store at -20°C. Avoid freeze-thaw cycles.