LSBio
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA and Assay Kits
  • All Kits
  • Assay Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Immunohistochemistry Reagents
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Reviews
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Anti-NUB1 Antibody (clone 2G1) LS-C175171

Catalog Size Price
LS-C175171-100 100 µl (1 mg/ml) Unavailable

Related Products

25 NUB1 Antibodies

Most Popular NUB1 Antibodies

Anti-NUB1 Antibody (aa566-615) LS-C118607
Rabbit Polyclonal (IgG) to Human NUB1
Human, Mouse
IHC, Western blot, ELISA
Unconjugated
Western blot of extracts from COS7 cells, using NYREN18 Antibody. The lane on the right is treated with the synthesized peptide.
Anti-NUB1 Antibody (clone 2G1) LS-C175171
Mouse Monoclonal [clone 2G1] (IgG1) to Human NUB1
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NUB1 mouse monoclonal antibody.
Anti-NUB1 Antibody (clone 4H2) LS-C175173
Mouse Monoclonal [clone 4H2] (IgG2a) to Human NUB1
Human, Monkey, Rat, Dog
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NUB1 mouse monoclonal antibody.
Anti-NUB1 Antibody (aa513-542) LS-C328085
Rabbit Polyclonal (IgG) to Human NUB1
Human
IHC, Western blot, ELISA
Unconjugated
Anti-NUB1 Antibody (aa87-117) LS-C328086
Rabbit Polyclonal (IgG) to Human NUB1
Human, Mouse
IHC, Western blot, ELISA
Unconjugated

100% Guaranteed
Mouse Monoclonal [clone 2G1] (IgG1) to Human NUB1
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human NUB1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2G1]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

NUB1 antibody was raised against human recombinant protein fragment corresponding to amino acids 1-326 of human NUB1 (NP_057202) produced in E. coli.
Human NUB1

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About NUB1

Q9Y5A7 NM_016118 NP_057202.3

NUB1 Antibody, BS4 Antibody, NEDD8 ultimate buster 1 Antibody, NEDD8 ultimate buster-1 Antibody, NUB1L Antibody, NYREN18 Antibody

NUB1 is a protein that functions as a negative regulator of NEDD8, a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by this gene regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NUB1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NUB1 mouse monoclonal antibody.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-NUB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot of extracts (35 ug) from 9 different cell lines by using g anti-NUB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUB1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NUB1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NUB1 antibody, and then analyzed by flow cytometry.

Requested From: 
Date Requested: 4/24/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number