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Catalog Number Size Price
LS-C60105-100 100 µg (1 mg/ml) $567 
NTRK3 / TRKC Antibody - Anti-TrkCT1 Antibody - Western Blot. Western blot of affinity purified anti-TrkCT1 to detect endogenous TrkCT1 in mouse cortex lysate (Lane 1). Lane 2 is TrkCT1 knock-out cortex lysate. Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:6000 dilution. Personal Communication, V. Coppola, CCRNCI, Frederick, MD.
NTRK3 / TRKC Antibody - Anti-TrkCT1 Antibody - Immunoprecipitation/Western Blot. Mouse cortex lysate was immunoprecipitated with anti-TrkCT1 antibody and further blotted with affinity purified anti-TrkCT1. Lane 1 is wild-type cortex lysate, Lane 2 is Tamalin knock-out cortex lysate, and Lane 3 is TrkCT1 knock-out cortex lysate. The membrane was probed with the primary antibody at a 1:6000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
NTRK3 / TRKC Antibody - Anti-TrkCT1 Antibody - Western Blot. Western blot of affinity purified anti-TrkCT1 to detect over-expressed TrkCT1 in HEK293 cells (Lane 2, arrowhead). Lane 1 is a non-transfected control. Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:3000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
NTRK3 / TRKC Antibody - Anti-TrkCT1 Antibody - Western Blot. Western blot of affinity purified anti-TrkCT1 to detect endogenous TrkCT1 in mouse cortex lysate (Lane 1). Lane 2 is TrkCT1 knock-out cortex lysate. Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:6000 dilution. Personal Communication, V. Coppola, CCRNCI, Frederick, MD.
NTRK3 / TRKC Antibody - Anti-TrkCT1 Antibody - Immunoprecipitation/Western Blot. Mouse cortex lysate was immunoprecipitated with anti-TrkCT1 antibody and further blotted with affinity purified anti-TrkCT1. Lane 1 is wild-type cortex lysate, Lane 2 is Tamalin knock-out cortex lysate, and Lane 3 is TrkCT1 knock-out cortex lysate. The membrane was probed with the primary antibody at a 1:6000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
NTRK3 / TRKC Antibody - Anti-TrkCT1 Antibody - Western Blot. Western blot of affinity purified anti-TrkCT1 to detect over-expressed TrkCT1 in HEK293 cells (Lane 2, arrowhead). Lane 1 is a non-transfected control. Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:3000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
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Polyclonal Rabbit anti‑Mouse NTRK3 / TRKC Antibody (WB) LS‑C60105

Polyclonal Rabbit anti‑Mouse NTRK3 / TRKC Antibody (WB) LS‑C60105

Antibody:
NTRK3 / TRKC Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C60105-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NTRK3 / TRKC Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
TRKC antibody LS-C60105 is an unconjugated rabbit polyclonal antibody to TRKC (NTRK3) from mouse. It is reactive with human, mouse and rat. Validated for ELISA, IP and WB.
Target
Mouse NTRK3 / TRKC
Synonyms
NTRK3 | ETV6-NTRK3 fusion | gp145-TrkC | Neurotrophin 3 receptor | TRKC | TrkC tyrosine kinase | NT-3 growth factor receptor | NT3 growth factor receptor | Tyrosine kinase receptor C | Pan Trk | gp145(trkC) | Trk-C
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Predicted
Human, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse TrkCT1 protein.
Specificity
A BLAST analysis was used to suggest cross-reactivity with TrkCT1 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
  • Western blot (1:1000 - 1:10000)
  • Immunoprecipitation
  • ELISA (1:5000 - 1:20000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. While the predicted molecular weight for TrkCT1 is approximately 70 kD, the protein has been reported to migrate at about 95 kD on western blots. The molecular weight noted for TrkCT1 depends somewhat upon the cell lysate or extract used for western blotting, and this variation may be due to post-translational modifications of the protein.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NTRK3 / TRKC
Q16288 NM_002530 NP_002521.2

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Requested From: United States
Date Requested: 4/18/2024