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Anti-NTRK1 / TrkA Antibody LS-C150092


Wt. Vol. Conc. Price
100 µg - - Unavailable

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100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human NTRK1 / TrkA
IHC, Western blot, Flow Cytometry, ELISA, Neutralization


Human NTRK1 / TrkA
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC (15 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • Flow Cytometry (3 - 10 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
  • Neutralization

Specificity and Use

NTRK1 / TrkA antibody was raised against nS0-derived rhTrkA extracellular domain.
Human TrkA.
Neutralization of Human TrkA bioactivity. The exact concentration of antibody required to neutralize cell surface TrkA mediated activity is dependent on cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the ligand-induced activity on a responsive cell line. The ND50 for this lot of anti-human TrkA antibody was determined to be approximately. 3-12 µg/ml. The P-NGF-dependent 3H-thymidine incorporation by TF-1 cells was used as an assay. Western blot-This antibody can be used at 0.1-0.2 µg/ml with the appropriate secondary reagents to detect human TrkA. The detection limit for rhTrkA is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry-This antibody will detect TrkA in cells and tissues. The working dilution is 15 µg/ml. Flow cytometry-This antibody can be used at 3-10 µg/ml/106 cells with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry. Direct ELISA-This antibody can be used at 0.5-1.0 µg/ml with the appropriate secondary reagents to detect human TrkA. The detection limit for rhTrkA is approximately 0.3 ng/well. In this format, this antibody shows approximately. 50% cross-reactivity with rrTrkA and 10% cross-reactivity with rhTrkB and rhTrKC.


Lyophilized from 0.2 µm filtered 100 mM NaHCO3, pH 8.3, 500 mM NaCl, 1% sucrose.
Store at -20°C.
For research use only.

About NTRK1 / TrkA

P04629 NM_002529 NP_002520.2

NTRK1 Antibody, Oncogene TRK Antibody, TrkA/NGF receptor Antibody, Tropomyosin-related kinase A Antibody, TRK Antibody, TRK1 Antibody, Tyrosine kinase receptor Antibody, Tyrosine kinase receptor A Antibody, gp140trk Antibody, MTC Antibody, p140-TrkA Antibody, Trk-A Antibody, TRKA Antibody

Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival.

Requested From: 
Date Requested: 4/23/2017

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