Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1D7] (IgG1) to Human NT5E / eNT / CD73
IHC - Paraffin, ELISA
Human NT5E / eNT / CD73
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [1D7]
IHC - Paraffin (1:200 - 1:400)
Specificity and Use
Purified recombinant fragment of NT5E expressed in E. coli.
Immunohistochemistry: LS-B3897 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3897 was determined to be 1:200.
Ascites fluid, 0.03% sodium azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
NT5E / eNT / CD73 is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.