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Anti-NT5E / eNT / CD73 Antibody (clone 1D7) IHC-plus™ LS-B3897

Note: This antibody replaces LS-C108849
Catalog Size Price
LS-B3897-50 50 µl Unavailable

Most Popular NT5E / eNT / CD73 Antibodies

Anti-NT5E / eNT / CD73 Antibody (aa29-549) LS-C73838
Sheep Polyclonal (IgG) to Mouse NT5E / eNT / CD73
Mouse
Western blot, Flow Cytometry, ELISA
Unconjugated
Anti-NT5E / eNT / CD73 Antibody (aa27-252, clone 2B6) IHC-plus™ LS-B2943
Mouse Monoclonal [clone 2B6] (IgG2b,k) to Human NT5E / eNT / CD73
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NT5E / eNT / CD73 Antibody (aa520-550) IHC-plus™ LS-B3428
Rabbit Polyclonal to Human NT5E / eNT / CD73
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NT5E / eNT / CD73 Antibody (clone 1D7) IHC-plus™ LS-B3897
Mouse Monoclonal [clone 1D7] (IgG1) to Human NT5E / eNT / CD73
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NT5E / eNT / CD73 Antibody (aa433-482) IHC-plus™ LS-B8284
Rabbit Polyclonal to Human NT5E / eNT / CD73
Human, Monkey, Mouse, Rat, Bat, Dog, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Mouse Monoclonal [clone 1D7] (IgG1) to Human NT5E / eNT / CD73
Human
IHC - Paraffin, ELISA
Unconjugated

Details

Human NT5E / eNT / CD73
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [1D7]
Unconjugated
Ascites
Unmodified

Applications

  • IHC - Paraffin (1:200 - 1:400)
  • ELISA (1:10000)

Specificity and Use

Purified recombinant fragment of NT5E expressed in E. coli.
Human NT5E
Immunohistochemistry: LS-B3897 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3897 was determined to be 1:200.

Packaging

Ascites fluid, 0.03% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About NT5E / eNT / CD73

P21589 NM_002526 NP_002517.1

NT5E Antibody, 5-nucleotidase, ecto (CD73) Antibody, 5'-nucleotidase Antibody, 5 nucleotidase (CD73) Antibody, 5-NT Antibody, 5-nucleotidase Antibody, 5'-nucleotidase, ecto (CD73) Antibody, CD73 Antibody, CD73 antigen Antibody, E5NT Antibody, Ecto-5-nucleotidase Antibody, ENT Antibody, EN Antibody, NT Antibody, Purine 5-Prime-Nucleotidase Antibody, NT5 Antibody, NTE Antibody

NT5E / eNT / CD73 is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.

Immunohistochemistry

Immunohistochemistry
Anti-NT5E / CD73 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3897 dilution 1:200.

Immunohistochemistry

Immunohistochemistry
Anti-NT5E / CD73 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3897 dilution 1:200.

Immunohistochemistry

Immunohistochemistry
Anti-NT5E / CD73 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3897 dilution 1:200.

Requested From: 
Date Requested: 8/21/2017

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