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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2943-50 | 50 µl (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human NT5E / eNT / CD73 Antibody (clone 2B6, aa27‑252, IHC, IF, WB) LS‑B2943
IHC‑plus™ Monoclonal Mouse anti‑Human NT5E / eNT / CD73 Antibody (clone 2B6, aa27‑252, IHC, IF, WB) LS‑B2943
Note: This antibody replaces LS-C93718
Antibody:
NT5E / eNT / CD73 Mouse anti-Human Monoclonal (aa27-252) (2B6) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA, SDS-PAGE
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
NT5E / eNT / CD73 Mouse anti-Human Monoclonal (aa27-252) (2B6) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA, SDS-PAGE
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CD73 antibody LS-B2943 is an unconjugated mouse monoclonal antibody to human CD73 (NT5E / eNT) (aa27-252). Validated for ELISA, IF, IHC, SDS-PAGE and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human NT5E / eNT / CD73
Synonyms
NT5E | 5-nucleotidase, ecto (CD73) | 5'-nucleotidase | 5 nucleotidase (CD73) | 5-NT | 5-nucleotidase | 5'-nucleotidase, ecto (CD73) | CD73 | CD73 antigen | E5NT | Ecto-5-nucleotidase | ENT | EN | NT | Purine 5-Prime-Nucleotidase | NT5 | NTE
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Clone
2B6
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human CD73 (27-252aa) purified from E. coli.
Epitope
aa27-252
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Immunofluorescence
- Western blot (1:1000 - 1:2000)
- ELISA
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2943 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2943 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NT5E / eNT / CD73
Validation
Anti-NT5E / CD73 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NT5E / CD73 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NT5E / CD73 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NT5E / CD73 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ NT5E / eNT / CD73 Antibody (aa27-252, clone 2B6) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B2943 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Elevated placental adenosine signaling contributes to the pathogenesis of preeclampsia. Iriyama T, Sun K, Parchim NF, Li J, Zhao C, Song A, Hart LA, Blackwell SC, Sibai BM, Chan LN, Chan TS, Hicks MJ, Blackburn MR, Kellems RE, Xia Y. Circulation. 2015 131:730-41. (IHC; Mouse)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, ELISA
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Mouse
Applications:
Western blot, Flow Cytometry, ELISA
Reactivity:
Human
Range:
1.56-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
