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Anti-Nsr1p Antibody (clone 31C4) LS-C143060


Wt. Vol. Conc. Price
- 500 µl - Unavailable

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Anti-Nsr1p Antibody (clone 31C4) LS-C143060
Mouse Monoclonal [clone 31C4] to Yeast Nsr1p
Immunofluorescence, Western blot
Anti-Nsr1p Antibody (clone 31C4) LS-C204568
Mouse Monoclonal [clone 31C4] (IgG1) to Yeast Nsr1p
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Mouse Monoclonal [clone 31C4] to Yeast Nsr1p
Immunofluorescence, Western blot


Yeast Nsr1p
Yeast (tested or 100% immunogen sequence identity)
Monoclonal [31C4]
Tissue culture supernatant


  • Immunofluorescence (1:500 - 1:5000)
  • Western blot (1:10000)

Specificity and Use

Nsr1p antibody was raised against nsr1p is localized in the nucleolus and binds to telomeric single-stranded oligonucleotide repeat sequence of the type and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport
31C4 was isolated during a screen for monoclonal antibodies reactive with yeast nucleolar proteins. We determined the specificity by screening a yeast genomic library expressed as b-galactosidase fusion proteins in lgt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones b-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with EFEH, corresponding to amino acids 190 and onwards in Nsr1p. This peptide is ~20 amino acids into the first RRM domain. Thus, MCA-31C4 reacts with an epitope in the C-terminal 55% of Nsr1p. The antibody is provided as sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The IgG isotype and exact concentration are not known.


Tissue culture supernatant, 10 mM sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About Nsr1p

Requested From: 
Date Requested: 4/25/2017

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