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Anti-NSPC1 / PCGF1 Antibody (aa143-192) LS-C116234

Catalog Size Price
LS-C116234-50 50 µg Unavailable

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10 NSPC1 / PCGF1 Antibodies

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Anti-NSPC1 / PCGF1 Antibody (aa143-192) LS-C116234
Rabbit Polyclonal to Human NSPC1 / PCGF1
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit, Zebrafish
Western blot
Unconjugated
PCGF1 antibody LS-C116234 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NSPC1 / PCGF1 Antibody (aa117-151) LS-C344663
Rabbit Polyclonal to Human NSPC1 / PCGF1
Human, Mouse, Rat
Western blot
Unconjugated
Western blot of lysates from human brain, mouse brain, mouse cerebellum, rat brain tissue (from left to right) with PCGF1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
Anti-NSPC1 / PCGF1 Antibody (aa132-167) LS-C356552
Rabbit Polyclonal to Human NSPC1 / PCGF1
Human
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-PCGF1 Antibody at 1:2000 dilution + K562 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-NSPC1 / PCGF1 Antibody LS-C412333
Rabbit Polyclonal to Human NSPC1 / PCGF1
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IHC, Western blot
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Anti-NSPC1 / PCGF1 Antibody LS-C672466
Rabbit Polyclonal (IgG) to Human NSPC1 / PCGF1
Human
Unconjugated
Immunohistochemistry image at a dilution of 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Rabbit Polyclonal to Human NSPC1 / PCGF1
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NSPC1 / PCGF1
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Guinea pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

NSPC1 / PCGF1 antibody was raised against synthetic peptide located between aa143-192 of human PCGF1 (Q9BSM1, NP_116062). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig (100%); Galago, Opossum, Guinea pig (92%); Bat (87%).
LS-E15955 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116234. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa143-192
Human PCGF1
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

About NSPC1 / PCGF1

Q9BSM1 NM_032673 NP_116062.2

PCGF1 Antibody, Polycomb group ring finger 1 Antibody, RNF3A-2 Antibody, Nervous system Polycomb-1 Antibody, NSPC1 Antibody, RING finger protein 68 Antibody, RNF68 Antibody

Component of the Polycomb group (PcG) multiprotein BCOR complex, a complex required to maintain the transcriptionally repressive state of some genes, such as BCL6 and the cyclin-dependent kinase inhibitor, CDKN1A. Transcriptional repressor that may be targeted to the DNA by BCL6; this transcription repressor activity may be related to PKC signaling pathway.

Western blot

PCGF1 antibody LS-C116234 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PCGF1 antibody LS-C116234 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/23/2018

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