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Anti-NRF1 / NRF-1 Antibody (aa360-409) IHC-plus™ LS-B3511

Note: This antibody replaces LS-C101504

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NRF1 / NRF-1 Antibodies

Anti-NRF1 / NRF-1 Antibody (aa360-409) IHC-plus™ LS-B3511
Rabbit Polyclonal (IgG) to Human NRF1 / NRF-1
Human, Monkey, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NRF1 / NRF-1 Antibody (aa1-534) IHC-plus™ LS-B43
Rabbit Polyclonal to Mouse NRF1 / NRF-1
Mouse, Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human NRF1 / NRF-1
Human, Monkey, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NRF1 / NRF-1
Rabbit
Human, Monkey, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Mouse
IgG Polyclonal
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

NRF1 / NRF-1 antibody was raised against synthetic peptide located between aa360-409 of human NRF1 (Q16656, NP_005002). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%).
LS-E12251 - Lyophilized - 100 µg - $145.00
aa360-409
Human NRF1
Immunohistochemistry: LS-B3511 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3511 was determined to be 10 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NRF1 / NRF-1

Q16656 NM_005011 NP_005002.3

NRF1 Antibody, EWG Antibody, NRF-1 Antibody, Nuclear respiratory factor 1 Antibody, ALPHA-PAL Antibody

NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles. In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene.

Immunohistochemistry

Immunohistochemistry
Anti-NRF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3511 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-NRF1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3511 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
NRF1 antibody LS-B3511 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/9/2016

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