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Anti-NRBP1 / NRBP Antibody (clone 6C11) LS-C114546

Ordering

Wt. Vol. Conc. Price
- 100 µl - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NRBP1 / NRBP Antibodies

Anti-NRBP1 / NRBP Antibody (C-Terminus) IHC-plus™ LS-B7408
Goat Polyclonal to Human NRBP1 / NRBP
Human, Rat, Bovine, Dog, Hamster, Horse, Rabbit
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NRBP1 / NRBP Antibody (clone 2C8) IHC-plus™ LS-B9403
Mouse Monoclonal [clone 2C8] (IgG2a) to Human NRBP1 / NRBP
Human
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 6C11] (IgG2a) to Human NRBP1 / NRBP
Human, Monkey
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human NRBP1 / NRBP
Mouse
Human, Monkey (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [6C11]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Western blot (1:200 - 1:500)
  • Immunoprecipitation
  • Flow Cytometry (1:100)

Specificity and Use

NRBP1 / NRBP antibody was raised against protein expressed in 293T cell transfected with human NRBP1 expression vector.
Human NRBP1 / MADM
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About NRBP1 / NRBP

Q9UHY1 NM_013392 NP_037524.1

NRBP1 Antibody, MUDPNP Antibody, NRBP Antibody, BCON3 Antibody, MADM Antibody

NRBP contains a kinase-homology domain and exhibits an associated kinase activity. In addition, NRBP is a multidomain putative adapter protein containing two putative nuclear receptor binding motifs, a putative binding domain for Src homology-2 domain containing proteins, a bipartite nuclear localization signal, and three sequences rich in glutamic acid, serine, proline, and threonine (PEST) residues.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded pancreas tissue using anti-NRBP1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded breast tissue using anti-NRBP1 mouse monoclonal antibody. (Dilution 1:50).

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1.

Western blot

Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-anti-NRBP1monoclonal antibody.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation(IP) of NRBP1 by using monoclonal anti-NRBP1 antibodies (Negative control: IP without adding anti-NRBP1 antibody.). For each experiment, 500ul of DDK tagged NRBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-NRBP1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NRBP1 antibody, and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 12/10/2016

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