Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The glucocorticoid receptor (GR), a NR3 Steroid Receptor, mediates the physiological activities of glucocorticoids that modulate development, homeostasis, stress, inflammatory, and immunologic responses. GR has been shown to mediate negative-feedback signals of elevated glucocorticoid levels; impaired GR signaling is a key mechanism in the pathogenesis of stress-related psychiatric disorders such as depression and anxiety.
NR3C1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Fluorescent confocal image of HeLa cell stained with NR3C1 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR3C1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NR3C1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
NR3C1 Antibody western blot of mouse liver tissue lysates (35 ug/lane). The NR3C1 antibody detected NR3C1 protein (arrow).
Requested From: United States
Date Requested: 10/26/2016