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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NR1D2 Antibody (aa503‑552) LS‑C109821
NR1D2 antibody LS-C109821 is an unconjugated rabbit polyclonal antibody to NR1D2 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C109821-50
50 µg (1 mg/ml)
Unavailable

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A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus
Applications: Western blot

Product Description

NR1D2 antibody LS-C109821 is an unconjugated rabbit polyclonal antibody to NR1D2 from human, mouse, rat and other species. Validated for WB.
About NR1D2
Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARNTL/BMAL1 and CLOCK. Also regulates genes involved in metabolic functions, including lipid metabolism and the inflammatory response. Q14995 D16815 BAA20088.1

NR1D2 Antibody, EAR-1R Antibody, HZF2 Antibody, Nuclear receptor rvr Antibody, Rev-erbabeta Antibody, Rev-erb-beta Antibody, Rev-erba beta Antibody, RVR Antibody, BD73 Antibody, Nuclear receptor ear-1r Antibody


Specifications

Target
Human NR1D2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
NR1D2 antibody was raised against synthetic peptide located between aa503-552 of human NR1D2 (Q14995, NP_005117). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Zebrafish (100%); Elephant (92%).
Epitope
aa503-552
Specificity
Human NR1D2
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

NR1D2 antibody LS-C109821 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NR1D2 antibody LS-C109821 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

NR1D2 antibody LS-C109821 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NR1D2 antibody LS-C109821 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

NR1D2 antibody LS-C109821 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NR1D2 antibody LS-C109821 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

NR1D2 antibody LS-C109821 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NR1D2 antibody LS-C109821 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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