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Anti-NPY1R Antibody (aa180-229) IHC-plus™ LS-B3546

Note: This antibody replaces LS-C80462

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NPY1R Antibodies

Anti-NPY1R Antibody (Extracellular Domain) IHC-plus™ LS-A1064
Rabbit Polyclonal to Human NPY1R
Human, Monkey
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human NPY1R
Human, Monkey, Mouse, Rat, Horse
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NPY1R
Rabbit
Human, Monkey, Mouse, Rat, Horse (tested or 100% immunogen sequence identity)
Bovine, Dog, Guinea pig, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

NPY1R antibody was raised against synthetic peptide located between aa180-229 of human NPY1R (P25929, NP_000900). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%); Galago, Sheep, Elephant, Panda, Dog, Bovine, Rabbit, Pig, Guinea pig (92%); Hamster (85%); Mouse, Rat (78%).
LS-E15387 - Lyophilized - 100 µg - $145.00
aa180-229
Human NPY1R
Immunohistochemistry: LS-B3546 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3546 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NPY1R

P25929 nm_000909 NP_000900.1

NPY1R Antibody, NPYY1 Antibody, NPY-Y1 receptor Antibody, Neuropeptide Y receptor type 1 Antibody, PY receptor Y1 Antibody, Neuropeptide Y receptor Y1 Antibody, Neuropeptide Y-Y1 receptor Antibody, Neuropeptide Y1 receptor Antibody, NPY1-R Antibody, NPYR Antibody

NPY1R belongs to the G-protein-coupled receptor superfamily. The encoded transmembrane protein mediates the function of neuropeptide Y (NPY), a neurotransmitter, and peptide YY (PYY), a gastrointestinal hormone. The encoded receptor undergoes fast agonist-induced internalization through clathrin-coated pits and is subsequently recycled back to the cell membrane. Activation of Y1 receptors may result in mobilization of intracellular calcium and inhibition of adenylate cyclase activity.

Immunohistochemistry

Immunohistochemistry
Anti-NPY1R antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3546 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-NPY1R antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3546 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/8/2016

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