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Anti-NPM1 / NPM / Nucleophosmin Antibody (aa81-294, clone 5E3) IHC-plus™ LS-B2930

Note: This antibody replaces LS-C93771
Catalog Size Price
LS-B2930-50 50 µg (1 mg/ml) Unavailable

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Formalin-fixed, paraffin-embedded human tonsil stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphocytes. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-NPM1 / NPM / Nucleophosmin Antibody (aa81-294, clone 5E3) IHC-plus™ LS-B2930
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Immunofluorescence of monoclonal antibody to NPM1 on HeLa cell. [antibody concentration 10 ug/ml]
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100% Guaranteed
Mouse Monoclonal [clone 5E3] (IgG2b,k) to Human NPM1 / NPM / Nucleophosmin
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human NPM1 / NPM / Nucleophosmin
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [5E3]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

Recombinant human NPM (81-294aa) purified from E. coli.
aa81-294
Immunohistochemistry: LS-B2930 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2930 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About NPM1 / NPM / Nucleophosmin

P06748 NM_002520 NP_002511.1

NPM1 Antibody, NPM Antibody, Nucleolar phosphoprotein B23 Antibody, Nucleolar protein NO38 Antibody, Numatrin Antibody, B23 Antibody, Nucleophosmin Antibody

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4.

Immunohistochemistry

Anti-NPM1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2930 concentration 5 ug/ml.
Anti-NPM1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2930 concentration 5 ug/ml.

Immunohistochemistry

Anti-NPM1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2930 concentration 5 ug/ml.
Anti-NPM1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2930 concentration 5 ug/ml.

Western blot

The HeLa and HepG2 cell lysates (5 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The HeLa and HepG2 cell lysates (5 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Requested From: 
Date Requested: 5/23/2018

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