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Anti-NPHS1 / Nephrin Antibody (C-Terminus) IHC-plus™ LS-B1382

Note: This antibody replaces LS-C19446

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NPHS1 / Nephrin Antibodies

Anti-NPHS1 / Nephrin Antibody (C-Terminus) IHC-plus™ LS-B1382
Rabbit Polyclonal to Human NPHS1 / Nephrin
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NPHS1 / Nephrin Antibody (aa1198-1227, Biotin) LS-C264204
Rabbit Polyclonal (IgG) to Human NPHS1 / Nephrin
Human, Mouse
Western blot, Flow Cytometry, ELISA
Biotin Conjugated
Anti-NPHS1 / Nephrin Antibody (aa1198-1227, FITC) LS-C264206
Rabbit Polyclonal (IgG) to Human NPHS1 / Nephrin
Human, Mouse
Western blot, Flow Cytometry, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NPHS1 / Nephrin
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human NPHS1 / Nephrin
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

NPHS1 / Nephrin antibody was raised against synthetic peptide from human NPHS1 / Nephrin.
LS-E29932 - Liquid - 50 µg - $145.00
C-Terminus
14 amino acid peptide from near the carboxy terminus of human Nephrin.
Immunohistochemistry: LS-B1382 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1382 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About NPHS1 / Nephrin

O60500 NM_004646 NP_004637.1

NPHS1 Antibody, Nephrin Antibody, NPHN Antibody, CNF Antibody

NPHS1 / Nephrin is a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine.

Immunohistochemistry

Immunohistochemistry
Anti-Nephrin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1382 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of Nephrin in Mouse Kidney cells with Nephrin antibody at 10 ug/ml.

Western blot

Western blot
Western blot of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
 

Requested From: United States
Date Requested: 12/10/2016

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