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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NPFFR1 / GPR147 Antibody (aa1‑16) IHC‑plus™ LS‑B261
Note: This antibody replaces LS-C2735
GPR147 antibody LS-B261 is an unconjugated rabbit polyclonal antibody to human GPR147 (NPFFR1). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B261-100
100 µg (1 mg/ml)
$425
Description
GPR147 antibody LS-B261 is an unconjugated rabbit polyclonal antibody to human GPR147 (NPFFR1). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human NPFFR1 / GPR147
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NPFFR1 / GPR147 antibody was raised against n terminal sequence MEGEPSQPPNSSWPLS and the C terminal sequence CSHLPLTIPAWDI of the human NPFF1 protein. Percent identity by BLAST analysis: Human, Gibbon (100%); Gorilla, Monkey, Marmoset (88%); Rat (81%).
Epitope
aa1-16
Specificity
Reacts with the N terminal sequence MEGEPSQPPNSSWPLS and the C terminal sequence CSHLPLTIPAWDI of the human NPFF1 protein.
Usage
Immunohistochemistry: LS-B261 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B261 was determined to be 2.5 ug/ml.
Presentation
PBS, 0.02% Sodium Azide
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only.
About NPFFR1 / GPR147
Q9GZQ6 NM_022146 NP_071429.1

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Anti-NPFFR1 / GPR147 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey, Bovine, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin
Anti-NPFFR1 / GPR147 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey, Bovine, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin

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Immunohistochemistry

Anti-NPFF1 Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NPFF1 Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-NPFF1 Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NPFF1 Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-NPFF1 Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NPFF1 Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-NPFF1 Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NPFF1 Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Requested From: United States
Date Requested: 12/13/2018
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