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| Catalog Number | Size | Price |
|---|---|---|
| LS-B88-0.1 | 0.1 ml (1 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Rat NPC1L1 Antibody (aa1000‑1100, IHC, IF, WB) LS‑B88
IHC‑plus™ Polyclonal Rabbit anti‑Rat NPC1L1 Antibody (aa1000‑1100, IHC, IF, WB) LS‑B88
Note: This antibody replaces LS-C2817
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
NPC1L1 antibody LS-B88 is an unconjugated rabbit polyclonal antibody to NPC1L1 (aa1000-1100) from rat. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Cited in 1 publication.
Target
Rat NPC1L1
Synonyms
NPC1L1 | Niemann-Pick C1-like protein 1 | NPC11L1 | NPC1-like 1
Host
Rabbit
Reactivity
Rat, Human, Mouse, Pig
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide made to an internal region of rat NPC1L1 (between residues 1000-1100).
Epitope
aa1000-1100
Specificity
NPC1L1 protein.
Applications
- IHC
- IHC - Paraffin (20 µg/ml)
- ICC
- Immunofluorescence
- Western blot (1:500)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B88 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B88 was determined to be 20 ug/ml.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.1% sodium azide.
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NPC1L1
Validation
Anti-NPC1L1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NPC1L1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ NPC1L1 Antibody (aa1000-1100) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B88 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Genetic demonstration of intestinal NPC1L1 as a major determinant of hepatic cholesterol and blood atherogenic lipoprotein levels. Xie P, Zhu H, Jia L, Ma Y, Tang W, Wang Y, Xue B, Shi H, Yu L. Atherosclerosis. Dec;237:609-17.
Customer Reviews (0)
Featured Products
Species:
Rat, Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Rat, Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Reactivity:
Human
Range:
40-10000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
