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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NPC / NPC1 Antibody (C‑Terminus, Biotin) LS‑C142211
NPC1 antibody LS-C142211 is a biotin-conjugated rabbit polyclonal antibody to NPC1 (NPC) from human, mouse and hamster. Validated for IF, IHC, IP and WB.
Catalog
Size
Price
LS-C142211-100
100 µl
Unavailable

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Product Description

NPC1 antibody LS-C142211 is a biotin-conjugated rabbit polyclonal antibody to NPC1 (NPC) from human, mouse and hamster. Validated for IF, IHC, IP and WB.
About NPC / NPC1
Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NM_000271

NPC1 Antibody, NPC Antibody, Niemann-Pick C1 protein Antibody, Niemann-Pick disease, type C1 Antibody


Specifications

Target
Human NPC / NPC1
Host
Rabbit
Reactivity
Human, Mouse, Hamster (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with DY650, DY488, DY550.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:250)
  • IHC - Paraffin
  • Immunofluorescence (1:250)
  • Western blot (1:1000 - 1:3000)
  • Immunoprecipitation (1:1000 - 1:3000)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NPC / NPC1 antibody was raised against a synthetic peptide made to the C-terminal region of human NPC1
Epitope
C-Terminus
Specificity
NPC1 protein.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-NPC1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B509 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NPC1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B509 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human NPC1 in 20 ug of human fibroblast cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human NPC1 in 20 ug of human fibroblast cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human NPC1 in 20 ug of human fibroblast cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human NPC1 in 20 ug of human fibroblast cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-NPC1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B509 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NPC1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B509 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human NPC1 in 20 ug of human fibroblast cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human NPC1 in 20 ug of human fibroblast cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human NPC1 in 20 ug of human fibroblast cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human NPC1 in 20 ug of human fibroblast cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-NPC1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B509 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NPC1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B509 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human NPC1 in 20 ug of human fibroblast cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human NPC1 in 20 ug of human fibroblast cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human NPC1 in 20 ug of human fibroblast cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human NPC1 in 20 ug of human fibroblast cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-NPC1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B509 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NPC1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B509 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human NPC1 in 20 ug of human fibroblast cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human NPC1 in 20 ug of human fibroblast cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human NPC1 in 20 ug of human fibroblast cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human NPC1 in 20 ug of human fibroblast cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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