Human NOXO1

Rabbit

Human, Chimpanzee (tested or 100% immunogen sequence identity)

Human, Chimpanzee (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

NOXO1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 238-252 of human NOXO1 protein.

A BLAST analysis was used to suggest cross reactivity with NOXO1 protein from human and chimpanzee (100% homology). Expect reactivity with alpha, delta and gamma isoforms of NOXO1. Also expect partial reactivity against NOXO1 homologues from dog and guinea pig (87%), as well as rat (75%) and mouse (68%). Reactivity against homologues from other sources is not known.

• IHC
• IHC - Paraffin (5 - 10 µg/ml)
• Western blot (1:1000 - 1:10000)
• ELISA (1:20000 - 1:100000)

Immunohistochemistry: LS-B485 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B485 was determined to be 5-10 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q8NFA2 NCBI: NM_144603 NP_751908.1