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Anti-NOVA1 Antibody (aa121-134) IHC-plus™ LS-B3259

Note: This antibody replaces LS-C61773
Catalog Size Price
LS-B3259-50 50 µg (0.5 mg/ml) Unavailable

Related Products

17 NOVA1 Antibodies

Most Popular NOVA1 Antibodies

Anti-NOVA1 Antibody (C-Terminus) LS-C80324
Rabbit Polyclonal (IgG) to Human NOVA1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus
Western blot
Unconjugated
Hela cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NOVA1 Antibody (aa121-134) IHC-plus™ LS-B3259
Goat Polyclonal to Human NOVA1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Pig, Rabbit, Chicken, Xenopus
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-NOVA1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3259 concentration 5 ug/ml.
Anti-NOVA1 Antibody (clone 5D9) LS-C139334
Mouse Monoclonal [clone 5D9] (IgG1,k) to Human NOVA1
Human, Rat
Western blot, ELISA
Unconjugated
NOVA1 monoclonal antibody (M10), clone 5D9. Western blot of NOVA1 expression in PC-12.
Anti-NOVA1 Antibody (C-Terminus) IHC-plus™ LS-B12455
Rabbit Polyclonal (IgG) to Human NOVA1
Human
IHC, IHC - Paraffin, ICC, Western blot
Unconjugated
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-NOVA1 Antibody LS-C663875
Rabbit Polyclonal to Human NOVA1
Human
IHC, Western blot
Unconjugated

100% Guaranteed Publications (2)
Goat Polyclonal to Human NOVA1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Pig, Rabbit, Chicken, Xenopus
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human NOVA1
Goat
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Pig, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.01 - 0.03 µg/ml)
  • ELISA (1:64000)

Specificity and Use

NOVA1 antibody was raised against synthetic peptide C-REMPQNVAKTEPVS from an internal region of human NOVA1 (NP_002506.2 ; NP_006480.2 ; NP_006482.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus (100%); Horse (86%).
LS-E26643 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3259. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa121-134
Human NOVA1. This antibody is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1)
Immunohistochemistry: LS-B3259 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3259 was determined to be 2.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

Publications (2)

1
High expression of neuro-oncological ventral antigen 1 correlates with poor prognosis in hepatocellular carcinoma. Zhang YA, Zhu JM, Yin J, Tang WQ, Guo YM, Shen XZ, Liu TT. PloS one. 2014 9:e90955. (WB; Human) [Full Text Article] [PubMed:24608171] [PMC:PMC3946567]
2
RNA binding protein Nova1 promotes tumor growth in vivo and its potential mechanism as an oncogene may due to its interaction with GABAA Receptor-?2. Zhang YA, Liu HN, Zhu JM, Zhang DY, Shen XZ, Liu TT. Journal of biomedical science. 2016 23:71. (WB; Human) [Full Text Article] [PubMed:27733149] [PMC:PMC5062898]

About NOVA1

P51513 NM_002515 NP_002506.2

NOVA1 Antibody, Nova-1 Antibody, Onconeural ventral antigen 1 Antibody, RNA-binding protein Nova-1 Antibody, Paraneoplastic Ri antigen Antibody

NOVA1 is a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described.

Immunohistochemistry

Anti-NOVA1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3259 concentration 5 ug/ml.
Anti-NOVA1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3259 concentration 5 ug/ml.

Immunohistochemistry

Anti-NOVA1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3259 concentration 5 ug/ml.
Anti-NOVA1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3259 concentration 5 ug/ml.

Western blot

HEK293 overexpressing NOVA1 (RC210407) and probed with (mock transfection in first lane).
HEK293 overexpressing NOVA1 (RC210407) and probed with (mock transfection in first lane).

Western blot

Antibody (0.01 ug/ml) staining of Human Breast Cancer lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.01 ug/ml) staining of Human Breast Cancer lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 4/23/2018

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