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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NOTCH4 Antibody (aa1778‑2003) LS‑C37775
Antibody
NOTCH4 antibody LS-C37775 is an unconjugated rat monoclonal antibody to human NOTCH4. Validated for ELISA, ICC and WB.
Catalog
Size
Price
LS-C37775-100
100 µg
Unavailable

Popular NOTCH4 Products

Antibody
Species: Mouse
Applications: Western blot
Anti-NOTCH4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3498 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Bovine, Guinea pig, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot
Staining of CD8+CD11c+-gated C57Bl/6 splenocytes with 0.25 ug of PE Armenian Hamster IgG isotype control (blue histogram) or 0.5 ug of PE anti-mouse Notch4 (HMN4-14) (purple histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Flow Cytometry
Immunohistochemistry Image
Species: Human
Applications: IHC, ELISA
Anti-NOTCH4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7289 dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA

Product Description

NOTCH4 antibody LS-C37775 is an unconjugated rat monoclonal antibody to human NOTCH4. Validated for ELISA, ICC and WB.
About NOTCH4
NOTCH4 is a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. Q99466 NM_004557 NP_004548.3

NOTCH4 Antibody, HNotch4 Antibody, Notch (Drosophila) homolog 4 Antibody, Notch homolog 4 (Drosophila) Antibody, Notch homolog 4 Antibody, INT3 Antibody, Notch 4 Antibody


Specifications

Target
Human NOTCH4
Host
Rat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ICC (10 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
Immunogen
NOTCH4 antibody was raised against e. coli-derived recombinant human Notch-4 Intracellular Domain (aa1778-2003) Accession # Q99466.
Epitope
aa1778-2003
Specificity
Recognizes human Notch4, Intracellular Domain. Species sequence homology: to mouse 78%.
Usage
Suitable for use in Immunocytochemistry, ELISA and Western Blot. Immunocytochemistry: 10 ug/ml. ELISA (Direct): 0.5-1 ug/ml. Detection limit ~2 ng/well. Western Blot: 1-2 ug/ml. Detection limit ~5 ng/lane under non-reducing and reducing conditions.
Presentation
Lyophilized.
Reconstitution
100 µl deionized water
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/24/2018

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