Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Hamster Monoclonal [clone HMN4-14] (IgG) to Mouse NOTCH4
Rat Monoclonal (IgG2a) to Human NOTCH4
ICC, Western blot, ELISA
Human (tested or 100% immunogen sequence identity)
Protein G purified
ICC (10 µg/ml)
Western blot (1 - 2 µg/ml)
ELISA (0.5 - 1 µg/ml)
Specificity and Use
NOTCH4 antibody was raised against e. coli-derived recombinant human Notch-4 Intracellular Domain (aa1778-2003) Accession # Q99466.
Recognizes human Notch4, Intracellular Domain. Species sequence homology: to mouse 78%.
Suitable for use in Immunocytochemistry, ELISA and Western Blot. Immunocytochemistry: 10 ug/ml. ELISA (Direct): 0.5-1 ug/ml. Detection limit ~2 ng/well. Western Blot: 1-2 ug/ml. Detection limit ~5 ng/lane under non-reducing and reducing conditions.
NOTCH4 is a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions.