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Catalog Number Size Price
LS-C154837-100 100 µg (0.5 mg/ml) $503 
NOTCH4 Antibody - NOTCH4 antibody immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
NOTCH4 Antibody - NOTCH4 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
NOTCH4 Antibody - NOTCH4 antibody immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
NOTCH4 Antibody - NOTCH4 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Polyclonal Goat anti‑Human NOTCH4 Antibody (aa1261‑1273, IF) LS‑C154837

Polyclonal Goat anti‑Human NOTCH4 Antibody (aa1261‑1273, IF) LS‑C154837

Antibody:
NOTCH4 Goat anti-Human Polyclonal (aa1261-1273) Antibody
Application:
IF, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C154837-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NOTCH4 Goat anti-Human Polyclonal (aa1261-1273) Antibody
Application:
IF, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NOTCH4 antibody LS-C154837 is an unconjugated goat polyclonal antibody to NOTCH4 (aa1261-1273) from human. It is reactive with human, mouse and rat. Validated for IF and Peptide-ELISA.
Target
Human NOTCH4
Synonyms
NOTCH4 | HNotch4 | Notch (Drosophila) homolog 4 | Notch homolog 4 (Drosophila) | Notch homolog 4 | INT3 | Notch 4
Host
Goat
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence DHFHNGHCEKGCNN, from the internal region of the protein sequence according to NP_004548.3.
Epitope
aa1261-1273
Specificity
Human NOTCH4. The immunizong peptide represents part of the extracellular domain.
Applications
  • Immunofluorescence (10 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Preliminary experiments gave an approx 27kD band in all human, mouse and rat tissue and cell line lysates tested after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 210kD according to NP_004548.3. The 27kD band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOTCH4
Q99466 NM_004557 NP_004548.3

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/15/2024