(tested or 100% immunogen sequence identity)
- Immunofluorescence (1:400)
- Western blot
- Immunoprecipitation (1:200)
NOTCH2 antibody was raised against synthetic peptide corresponding residues surrounding Val2332 of human Notch2.
Endogenous levels of total Notch2. Species Crossreactivity: rat.
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
For research use only.
This antibody carries the LSBio 100% Guarantee.