Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey, Bat, Bovine, Dog
(tested or 100% immunogen sequence identity)
Mouse, Hamster, Rabbit (at least 90% immunogen sequence identity)
IHC - Paraffin (1:250)
Western blot (1:200 - 1:1000)
ELISA (1:15000 - 1:45000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
NOTCH2 antibody was raised against a synthetic peptide C-NAAERTPSHSGHLQ corresponding to amino acid residues 2396-2409 of human Notch 2, intracellular domain. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Spider monkey, Elephant, Panda, Baboon, Porcine, Bovine, Dog, Bat, Tamarin (100%); Mouse, Hamster, Rabbit, Opossum (93%); Rat (86%).
Recognizes human NOTCH 2. Species sequence homology: rat and mouse Notch 2-one amino acid difference.
Suitable for use in ELISA, Immunohistochemistry and Western Blot. ELISA: 1:15000-1:45000. Western Blot: 1:200-1:1000, Detects a band at ~110kD. Immunohistochemistry (FFPE): 1:250.
PBS, 0.01% sodium azide, before the addition of glycerol to 40%.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
NOTCH2 is a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions.