Human, Monkey, Bat, Bovine, Dog
(tested or 100% immunogen sequence identity)
Mouse, Hamster, Rabbit (at least 90% immunogen sequence identity)
- IHC - Paraffin (1:250)
- Western blot (1:200 - 1:1000)
- ELISA (1:15000 - 1:45000)
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including antigen retrieval solutions, blocking agents
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NOTCH2 antibody was raised against a synthetic peptide C-NAAERTPSHSGHLQ corresponding to amino acid residues 2396-2409 of human Notch 2, intracellular domain. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Spider monkey, Elephant, Panda, Baboon, Porcine, Bovine, Dog, Bat, Tamarin (100%); Mouse, Hamster, Rabbit, Opossum (93%); Rat (86%).
Recognizes human NOTCH 2. Species sequence homology: rat and mouse Notch 2-one amino acid difference.
Suitable for use in ELISA, Immunohistochemistry and Western Blot. ELISA: 1:15000-1:45000. Western Blot: 1:200-1:1000, Detects a band at ~110kD. Immunohistochemistry (FFPE): 1:250.
PBS, 0.01% sodium azide, before the addition of glycerol to 40%.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.