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Anti-NOTCH1 Antibody (N-Terminus) LS-C19109

Note: This antibody replaces LS-C73694
Catalog Size Price
LS-C19109-200 200 µl Unavailable

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100% Guaranteed Publications (3)
Rabbit Polyclonal to Human NOTCH1
Human, Mouse, Rat
Western blot, ELISA


Human NOTCH1
Human, Mouse, Rat (tested or 100% immunogen sequence identity)


  • Western blot
  • ELISA (1:20000 - 1:60000)

Specificity and Use

NOTCH1 antibody was raised against synthetic peptide from human NOTCH1.
Amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
This antibody is reactive with Notch 1 by western blotting. A strong response was also detected by ELISA against the immunizing peptide. This product was assayed by immunoblot against recombinant mouse Notch constructs in 293 cells. An 80 kD band corresponding to Notch 1 was observed at a 1:500 dilution. This product was also assayed against the peptide immunogen in a standard capture ELISA using Peroxidase conjugated anti-Rabbit IgG (H&L) [Goat] (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

Publications (3)

Aberrant activation of notch signaling in human breast cancer. Stylianou S, Clarke RB, Brennan K. Cancer research. 2006 66:1517-25. [PubMed:16452208]
Upregulation of the let-7 microRNA with precocious development in lin-12/Notch hypermorphic Caenorhabditis elegans mutants. Solomon A, Mian Y, Ortega-Cava C, Liu VW, Gurumurthy CB, Naramura M, Band V, Band H. Developmental biology. 2008 316:191-9. [PubMed:18334253] [PMC:PMC2390880]
Effect of Notch activation on the regenerative response to acute renal failure. Gupta S, Li S, Abedin MJ, Wang L, Schneider E, Najafian B, Rosenberg M. American journal of physiology. Renal physiology. 2010 298:F209-15. [PubMed:19828677]

About NOTCH1

P46531 NM_017617 NP_060087.3

NOTCH1 Antibody, Notch 1 Antibody, TAN1 Antibody

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Western blot

Western blot
Rabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot. Equivalent amounts of lysates from transiently transfected 293 cells expressing recombinant myc-tagged mouse Notch constructs were electrophoresed and transferred to membrane using standard methods. A reaction with diluted primary antibody was followed by washing; reaction with a 1:10000 dilution of HRP conjugated Gt-a-Rabbit IgG, and color development. Lane M: Mol wt markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.

Requested From: 
Date Requested: 1/22/2018

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