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Anti-NOTCH1 Antibody (aa2488-2502) IHC-plus™ LS-B398

Note: This antibody replaces LS-C19108, LS-C73699


Wt. Vol. Conc. Price
- 50 µl 75 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular NOTCH1 Antibodies

Anti-NOTCH1 Antibody (aa2488-2502) IHC-plus™ LS-B398
Rabbit Polyclonal to Human NOTCH1
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-NOTCH1 Antibody (clone 3E12) LS-C114369
Mouse Monoclonal [clone 3E12] (IgG2b) to Human NOTCH1
IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry Image
Anti-NOTCH1 Antibody (Val1754) LS-C178239
Rabbit Polyclonal to Human NOTCH1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human NOTCH1
IHC - Paraffin, Western blot, ELISA


Human NOTCH1
Human (tested or 100% immunogen sequence identity)


  • IHC - Paraffin (1:500)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)

Specificity and Use

NOTCH1 antibody was raised against synthetic peptide from human NOTCH1.
Amino acid residues 2488-2502 of human Notch 1. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunohistochemistry: LS-B398 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B398 was determined to be 1:500.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

Aberrant activation of notch signaling in human breast cancer. Stylianou S, Clarke RB, Brennan K. Cancer research. 2006 66:1517-25. [PubMed:16452208]

About NOTCH1

P46531 NM_017617 NP_060087.3

NOTCH1 Antibody, Notch 1 Antibody, TAN1 Antibody

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.


Anti-NOTCH1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B398 dilution 1:500.

Requested From: United States
Date Requested: 10/22/2016

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