Human NOTCH1

Sheep

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

E. coli-derived recombinant human rhNotch-1 (aa 2428 - 2556).

aa2428-2556

Recognizes human Notch-1 intracellular domain. Species sequence homology: canine 83% and mouse 89%. < 1% cross-reactivity with rhNotch-2 IC, rhNotch-3 IC, and rhNotch-4 IC.

• ICC (10 µg/ml)
• Western blot (0.1 - 0.2 µg/ml)
• Flow Cytometry (50 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in for use in Western blot, Immunocytochemistry, Flow cytometry. and Direct ELISA. Western blot: 0.1-0.2 ug/ml. The detection limit for rhNotch-1 is ~0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml to detect human Notch-1 in SAOS human osteosarcoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for. 3 hours at RT followed by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. Flow cytometry: 50 ug/ml and add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10il of a 25 ug/ml stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Notch-1. The detection limit is ~0.2 ng/well.

Lyophilized from PBS, 5% Trehalose

Reconstitute in sterile PBS to 0.2 mg/ml.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P46531 NCBI: NM_017617 NP_060087.3