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Anti-NOTCH1 Antibody (aa2428-2556) LS-C73692

Ordering

Wt. Vol. Conc. Price
100 µg 200 µl 0.5 mg/ml $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular NOTCH1 Antibodies

Anti-NOTCH1 Antibody (aa2488-2502) IHC-plus™ LS-B398
Rabbit Polyclonal to Human NOTCH1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NOTCH1 Antibody (clone 3E12) LS-C114369
Mouse Monoclonal [clone 3E12] (IgG2b) to Human NOTCH1
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NOTCH1 Antibody (Val1754) LS-C178239
Rabbit Polyclonal to Human NOTCH1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2a) to Human NOTCH1
Human
ICC, Western blot, ELISA
Unconjugated

Details

Human NOTCH1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Unconjugated
Protein A/G purified
Unmodified

Applications

  • ICC (10 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

NOTCH1 antibody was raised against e. coli-derived rhNotch-1 IC. (aa 2428 - 2556).
aa2428-2556
Recognizes human Notch-1 IC.
Suitable for use in Direct ELISA, Western Blot and Immunocytochemistry. Direct ELISA: 0.5-1.0 ug/ml detection limit for rhNotch-1 IC is ~3 ng/well. Western Blot: 1-2 ug/ml detection limit for rhNotch-1 IC is ~25 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml. For SAOS2 osteosarcoma cells, cells were fixed with PBS containing 4% paraformaldehyde for 20min at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100 and 1% BSA for 45 min at RT. After blocking, cells were incubated with primary antibody overnight at 4?C followed by fluorochrome-coupled anti-mouse IgG at RT in the dark for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA.

Packaging

Lyophilized, PBS, 5% trehalose
200 µl Sterile PBS
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About NOTCH1

P46531 NM_017617 NP_060087.3

NOTCH1 Antibody, Notch 1 Antibody, TAN1 Antibody

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Requested From: United States
Date Requested: 12/5/2016

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