LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-NOS3 / eNOS Antibody (phospho-Ser635) LS-C37763

Ordering

Wt. Vol. Conc. Price
- 200 µl - $635
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NOS3 / eNOS Antibodies

Anti-NOS3 / eNOS Antibody (aa1181-1194) IHC-plus™ LS-B5676
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Human, Monkey, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NOS3 / eNOS Antibody (aa597-611) IHC-plus™ LS-B6341
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NOS3 / eNOS Antibody (aa1156-1183, FITC) LS-C256689
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Human
ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Bovine NOS3 / eNOS
Bovine
Western blot
Unconjugated

Details

Bovine NOS3 / eNOS
Rabbit
Bovine (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

Western blot (1:2000 - 1:5000)

Specificity and Use

NOS3 / eNOS antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Ser635 of bovine eNOS/NOS III. This site corresponds to human Ser633. The immunizing sequence is identical in human and mouse, and has 11/12 identical amino acids in canine.
pSer635
Recognizes phosphorylated eNOS/NOS III (Ser635), Mr 132kD. A non-specific protein was also detected, Mr 160kD. Species cross-reactivity: Bovine. Predicted to cross-react with human, mouse and canine based on sequence homology.
Suitable for use in Western Blot. Western Blot: 1:2000-1:5000 detects phosphorylated eNOS/NOS III (Ser635) in RIPA lysates from fetal bovine heart endothelial (FBHE) cells. Lambda protein phosphatase treated and untreated FBHE cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho eNOS/NOS III (Ser635) (1:2000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
For research use only.

About NOS3 / eNOS

P29474 NM_000603 NP_000594.2

NOS3 Antibody, Constitutive NOS Antibody, CNOS Antibody, Endothelial NOS Antibody, ECNOS Antibody, NOS type III Antibody, NOSIII Antibody, EC-NOS Antibody, ENOS Antibody, Nitric oxide synthase 3 Antibody

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Requested From: United States
Date Requested: 12/3/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number