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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NOS3 / eNOS Antibody (phospho‑Ser617) LS‑C37762
Antibody
ENOS antibody LS-C37762 is an unconjugated rabbit polyclonal antibody to eNOS (NOS3) from human and bovine. Validated for ELISA and WB.
Catalog
Size
Price
LS-C37762-200
200 µl
Unavailable

Popular NOS3 / eNOS Products

Antibody
Species: Bovine, Human, Mouse, Rat, Dog, Guinea pig, Pig
Applications: Immunofluorescence, Western blot, ELISA
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Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5676 concentration 5 ug/ml.
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Anti-ENOS antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6341 dilution 1:200.
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Applications: IHC, IHC - Paraffin, ICC, Western blot
Antibody
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

ENOS antibody LS-C37762 is an unconjugated rabbit polyclonal antibody to eNOS (NOS3) from human and bovine. Validated for ELISA and WB.
About NOS3 / eNOS
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. P29474 NM_000603 NP_000594.2

NOS3 Antibody, Constitutive NOS Antibody, CNOS Antibody, Endothelial NOS Antibody, ECNOS Antibody, NOS type III Antibody, NOSIII Antibody, EC-NOS Antibody, ENOS Antibody, Nitric oxide synthase 3 Antibody


Specifications

Target
Human NOS3 / eNOS
Host
Rabbit
Reactivity
Human, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:2000)
  • ELISA
  • (applications tested for the base form of this product only)
Immunogen
NOS3 / eNOS antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Ser617 of eNOS/NOS III. This site corresponds to human Ser615.
Epitope
pSer617
Specificity
Recognizes bovine phosphorylated eNOS/NOS III (Ser617), Mr 132kD. A non-specific protein was also detected, Mr 70kD. Species sequence homology: human, mouse and canine.
Usage
Suitable for use in ELISA and Western Blot. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/23/2018

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