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Catalog Number Size Price
LS-C37760-100 100 µg $832 
Antibody

Polyclonal Rabbit anti‑Human NOS3 / eNOS Antibody (IHC, WB) LS‑C37760

Polyclonal Rabbit anti‑Human NOS3 / eNOS Antibody (IHC, WB) LS‑C37760

Antibody:
NOS3 / eNOS Rabbit anti-Human Polyclonal Antibody
Application:
IHC, ICC, WB, ELISA
Reactivity:
Human, Mouse, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$832
LS-C37760-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NOS3 / eNOS Rabbit anti-Human Polyclonal Antibody
Application:
IHC, ICC, WB, ELISA
Reactivity:
Human, Mouse, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
ENOS antibody LS-C37760 is an unconjugated rabbit polyclonal antibody to eNOS (NOS3) from human. It is reactive with human, mouse and bovine. Validated for ELISA, ICC, IHC and WB.
Target
Human NOS3 / eNOS
Synonyms
NOS3 | Constitutive NOS | CNOS | Endothelial NOS | ECNOS | NOS type III | NOSIII | EC-NOS | ENOS | Nitric oxide synthase 3
Host
Rabbit
Reactivity
Human, Mouse, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
15 AA peptide sequence corresponding to human eNOS. No significant sequence homology is seen with NOS-1/NOS-2 or other proteins. Species Sequence Homology: Mouse, bovine - 100% (15/15 AA); Rat - 93% (14/15 AA); Porcine - 86% (13/15 AA).
Specificity
Recognizes the 135-140kD protein human endothelial nitric oxide synthase (eNOS, NOS III).
Applications
  • IHC (5 - 10 µg/ml)
  • ICC
  • Western blot (1 - 10 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in ELISA, Western Blot and Immunocytochemistry, and Immunohistochemistry. Western Blot: 1-10 ug/ml; Recognizes 135-140kD protein. Immunocytochemistry/Immunohistochemistry: 5-10 ug/ml. Useful on tissue sections fixed with 3.5% paraformaldehyde. We recommend overnight incubation with antibody at 4?C and detection by ABC (peroxidase) technique.
Presentation
PBS, pH 7.5, 0.1% BSA, 40% glycerol.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOS3 / eNOS
P29474 NM_000603 NP_000594.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024