Target
Human NOS3 / eNOS
Synonyms
NOS3 | Constitutive NOS | CNOS | Endothelial NOS | ECNOS | NOS type III | NOSIII | EC-NOS | ENOS | Nitric oxide synthase 3
Reactivity
Human, Mouse, Bovine
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
15 AA peptide sequence corresponding to human eNOS, coupled to KLH. Human, rat, mouse and bovine eNOS/NOS-3 are about aa 1202-1205 proteins.
Specificity
Recognizes the 135-140kD protein endothelial nitric oxide synthase (eNOS, NOS III). Species cross-reactivity: 100% in mouse, bovine (15/15 aa). Species sequence homology: 93% in rat (14/15 aa) and 86% in porcine (13/15 aa). No significant sequence homology is seen with NOS-1/NOS-2 or other proteins.
Applications
- Western blot (1:1000 - 1:5000)
- ELISA (1 µg/ml)
Usage
Suitable for use in ELISA and Western Blot. ELISA: Control peptide should be coated at 1 ug/ml. Western Blot: 1:1000-1:5000. Recognizes 135-140kD protein.
Presentation
PBS, 0.05% Sodium Azide, 40% Glycerol
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee