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Antibodies > NOS2 / iNOS > LS-C352602
 
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Catalog Number Size Price
LS-C352602-100 100 µl (1 mg/ml) $299 
NOS2 / iNOS Antibody - Immunohistochemical analysis of iNOS staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NOS2 / iNOS Antibody - Immunofluorescent analysis of iNOS staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NOS2 / iNOS Antibody - Western blot analysis of iNOS expression in MCF7 (A); A549 (B); Raw264.7 (C); PC12 (D); rat liver (E) whole cell lysates.
NOS2 / iNOS Antibody - Immunohistochemical analysis of iNOS staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NOS2 / iNOS Antibody - Immunofluorescent analysis of iNOS staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NOS2 / iNOS Antibody - Western blot analysis of iNOS expression in MCF7 (A); A549 (B); Raw264.7 (C); PC12 (D); rat liver (E) whole cell lysates.
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Polyclonal Rabbit anti‑Human NOS2 / iNOS Antibody (C‑Terminus, IHC, IF, WB) LS‑C352602

Polyclonal Rabbit anti‑Human NOS2 / iNOS Antibody (C‑Terminus, IHC, IF, WB) LS‑C352602

Submit Review LSBio Guarantee
Antibody:
NOS2 / iNOS Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352602-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NOS2 / iNOS Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
INOS antibody LS-C352602 is an unconjugated rabbit polyclonal antibody to iNOS (NOS2) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human NOS2 / iNOS
Synonyms
NOS2 | Hepatocyte NOS | HEP-NOS | Inducible NOS | NOS, type II | NOS type II | Inducible NO synthase | NOS2A | INOS | NOS
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human iNOS.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of iNOS protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOS2 / iNOS
P35228 NM_000625 NP_000616.3

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NOS2 / iNOS Antibody - Western Blot; Sample: Recombinant protein.
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See all NOS2 / iNOS Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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