Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 37C12] (IgG1) to Yeast Nop5p + Nop6p
Yeast Nop5p + Nop6p
Yeast (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [37C12]
Tissue culture supernatant
Immunofluorescence (1:1000 - 1:10000)
Specificity and Use
Nop5p + Nop6p antibody does not work well for immunoblotting, though it does recognize both yeast Nop5p and Nop6p, both of which are nucleolar proteins. For immunoblotting of Nop5p we recommend the monoclonal. the antibody is however good for immunofluorescence on yeast cells, and we recommend dilutions in the range of 1:1000-1:10000. We routinely dilute to 1:2000. For immunoprecipitations, try 2-4 ul of the antibody with the amount of lysate derived from 5 OD600 units of yeast culture.
Tissue culture supernatant, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.