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Anti-NOG / Noggin Antibody (clone 4C12) LS-C114324

Ordering

Wt. Vol. Conc. Price
- 100 µl - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NOG / Noggin Antibodies

Anti-NOG / Noggin Antibody (Internal) IHC-plus™ LS-B2338
Rabbit Polyclonal (IgG) to Human NOG / Noggin
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NOG / Noggin Antibody (aa83-111) LS-C323390
Rabbit Polyclonal (IgG) to Human NOG / Noggin
Human, Mouse
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4C12] (IgG2b) to Human NOG / Noggin
Human, Dog
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human NOG / Noggin
Mouse
Human, Dog (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [4C12]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000 - 1:2000)

Specificity and Use

NOG / Noggin antibody was raised against human recombinant protein fragment corresponding to amino acids 28-232 of human noggin (NP_005441) produced in E. coli.
Human Noggin
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About NOG / Noggin

Q13253 NM_005450 NP_005441.1

NOG Antibody, Symphalangism 1 (proximal) Antibody, SYNS1 Antibody, SYM1 Antibody, Noggin Antibody

The secreted polypeptide, encoded by this gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages.

Immunofluorescence

Immunofluorescence
Immunofluorescent staining of A549 cells using anti-Nog mouse monoclonal antibody.

Immunofluorescence

Immunofluorescence
Anti-Nog mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Nog.

Immunofluorescence

Immunofluorescence
Immunofluorescent staining of A549 cells using anti-Nog mouse monoclonal antibody.

Immunofluorescence

Immunofluorescence
Anti-Nog mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Nog.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Noggin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Noggin.

Western blot

Western blot
Noggin antibody (4C12) at 1:2000 + Recombinant human Noggin.

Requested From: United States
Date Requested: 12/9/2016

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