LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
NODAL Antibody (clone 5C3) LS‑C133350
NODAL antibody LS-C133350 is an unconjugated mouse monoclonal antibody to NODAL from human, mouse and rat. Validated for ELISA, IF, IHC and WB.
Catalog
Size
Price
LS-C133350-100
100 µg (0.5 mg/ml)
Unavailable

Popular NODAL Products

Antibody
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Applications: Peptide Enzyme-Linked Immunosorbent Assay
Anti-NODAL antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3955 concentration 3.75 ug/ml.
Species: Human, Monkey, Bat, Bovine, Dog, Horse, Pig
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Anti-NODAL Antibody at 1:1000 dilution + HepG2 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemical analysis of NODAL staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

NODAL antibody LS-C133350 is an unconjugated mouse monoclonal antibody to NODAL from human, mouse and rat. Validated for ELISA, IF, IHC and WB.
About NODAL
Essential for mesoderm formation and axial patterning during embryonic development. Q96S42 NM_018055 NP_060525.3

NODAL Antibody, HTX5 Antibody, Nodal homolog Antibody, Nodal, mouse, homolog Antibody, Nodal homolog (mouse) Antibody


Specifications

Target
Human NODAL
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [5C3]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3 - 5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NODAL antibody was raised against nODAL (NP_060525, 275 a.a. ~ 347 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human NODAL
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Cell lysate, Recombinant protein.
Presentation
PBS, pH 7.4.
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]

Immunofluorescence

Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in Raw 264.7.
NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in Raw 264.7.

Western blot

NODAL monoclonal antibody (M03), clone 5C3 Western blot of NODAL expression in HeLa.
NODAL monoclonal antibody (M03), clone 5C3 Western blot of NODAL expression in HeLa.

Western blot

NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in PC-12.
NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in PC-12.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NODAL is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NODAL is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]

Immunofluorescence

Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in Raw 264.7.
NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in Raw 264.7.

Western blot

NODAL monoclonal antibody (M03), clone 5C3 Western blot of NODAL expression in HeLa.
NODAL monoclonal antibody (M03), clone 5C3 Western blot of NODAL expression in HeLa.

Western blot

NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in PC-12.
NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in PC-12.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NODAL is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NODAL is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]

Immunofluorescence

Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in Raw 264.7.
NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in Raw 264.7.

Western blot

NODAL monoclonal antibody (M03), clone 5C3 Western blot of NODAL expression in HeLa.
NODAL monoclonal antibody (M03), clone 5C3 Western blot of NODAL expression in HeLa.

Western blot

NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in PC-12.
NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in PC-12.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NODAL is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NODAL is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]

Immunofluorescence

Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in Raw 264.7.
NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in Raw 264.7.

Western blot

NODAL monoclonal antibody (M03), clone 5C3 Western blot of NODAL expression in HeLa.
NODAL monoclonal antibody (M03), clone 5C3 Western blot of NODAL expression in HeLa.

Western blot

NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in PC-12.
NODAL monoclonal antibody (M03), clone 5C3. Western blot of NODAL expression in PC-12.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NODAL is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NODAL is approximately 0.3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/25/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number